On the anniversary of the outstanding biologist, Academician of the Russian Academy of Sciences Mikhail Petrovich Kirpichnikov

This publication is dedicated to the 80th birthday of Mikhail P. Kirpichnikov, a prominent researcher in the sphere of physicochemical biology, bioengineering and biotechnology, a renowned educator and research coordinator. Key areas of his research activities are highlighted, including his pioneering inputs to protein engineering, and their importance for the development of genetics, breeding, bioeconomics, and nature-inspired technologies. Special attention is focused on this contribution to researcher training, government affairs, and civic service.

1. 80th anniversary of academician Mikhail Petrovich Kirpichnikov (80 let akademiku Mikhailu Petrovichu Kirpichnikovu). Lomonosov Moscow State University, Faculty of Biology: [website]. 2025. [in Russian]. URL: https://bio.msu.ru/2025/11/09/грандиозный-юбилей-80-лет-академику-мих/. Дата публикации: 09.11.2025.

2. Academician M. P. Kirpichnikov: “Nature is the chief genetic engineer”. Interlocutor: Elena Ponizovkina. The Yekaterinburg Public Scientific Demidov Foundation: [website]. 2023. [in Russian]. URL: https://demidov.uran.ru/article/akademik-m-p-kirpichnikov-glavnyj-gennyj-inzhener-priroda. Дата публикации: 08.02.2023.

3. Academician Mikhail Petrovich Kirpichnikov (Akademik Kirpichnikov Mikhail Petrovich). Russian Academy of Sciences: [website]. 2025. [in Russian]. URL: https://new.ras.ru/anniversary/akademik-kirpichnikov-mikhail-petrovich/ [дата обращения: 14.11.2025].

4. Afazizheva I.Yu., Dolgikh D.A., Kirpichnikov M.P. Study of immunogenic properties of albeferon, biologically active de novo protein. Molecular Biology. 1999;33(4):602-606.

5. Afonin D.A., Geras’kina O.V., Loseva T.V., Kirpichnikov M.P., Studitsky V.M., Feofanov A.V. Structure and affinity of complexes between the DNA-binding domain of Swi4 and DNA. Biophysics. 2022;67(5):700-707. DOI; 10.1134/S0006350922050037

6. Andreeva T., Maluchenko N., Chertkov O., Studitsky V., Feofanov A., Kirpichnikov M. Length of DNA linker affects nucleosomal DNA structure. Microscopy and Microanalysiss. 2020;26(S2):1390-1392. DOI; 10.1017/S1431927620017948

7. Armeev G.A., Kirpichnikov M.P., Kobelkov G.M., Kudryavtsev V., Lozhnikov M.A., Novoseletsky V.N., Shaitan A.K., Shaytan K.V. Application of neural networks for recognition of conformational changes in protein structure by X-ray diffractograms of its single molecules on the example of bacteriorhodopsin photocycle. Intelligent Systems. Theory and Applications. 2022;26(1):24-34. [in Russian]. URL: https://www.mathnet.ru/links/79455e25d6f3a6ffd2f67a040ce3175f/ista330.pdf [дата обращения: 10.11.2025].

8. Bocharov E.V., Mineev K.S., Volynsky P.E., Ermolyuk Y.S., Tkach E.N., Sobol A.G., Chupin V.V., Kirpichnikov M.P., Efremov R.G., Arseniev A.S., Spatial structure of the dimeric transmembrane domain of the growth factor receptor ErbB2 presumably corresponding to the receptor active state. Journal of Biological Chemistry. 2008;283(11):6950-6956. DOI: 10.1074/jbc.M709202200

9. Bychkov M.L.; Kirichenko A.V.; Shulepko M.A.; Mikhaylova I.N.; Kirpichnikov M.P.; Lyukmanova E.N. Mambalgin-2 inhibits growth, migration, and invasion of metastatic melanoma cells by targeting the channels containing an ASIC1a subunit whose up-regulation correlates with poor survival prognosis. Biomedicines. 2021:26;9(10):1324. DOI: 10.3390/biomedicines9101324

10. Demidov Prize laureate and RAS Academician M. P. Kirpichnikov: “Nature is the chief genetic engineer”. Russian Academy of Sciences: [website]. 2023. [in Russian]. URL: https://www.ras.ru/news/shownews.aspx?id=a7c2eb28-bca1-49c8-bff5-9c59fcaa8c2f. Дата публикации: 10.02.2023.

11. Dolgikh D.A., Kirpichnikov M.P., Ptitsyn O.B., Chemeris V.V. Protein engineering of artificial proteins. Molecular Biology. 1996a;30(2):261-272. [in Russian]

12. Dolgikh D.A., Kirpichnikov M.P., Ptitsyn O.B., Fedorov A.N., Finkelshtein A.V., Chemeris V.V. De novo proteins with a given spatial structure: new approaches to design and analysis (Belok de novo s zadannoy prostranstvennoy strukturoy: novye podkhody k konstruirovaniyu i analizu). Molecular Biology. 1992;26(6):1242-1250. [in Russian]

13. Dolgikh D.A., Uversky V.N., Gabrielian A.E., Chemeris V.V., Fedorov A.N., Navolotskaya E.V., Zav’yalov V.P. Kirpichnikov M.P. The de novo protein with grafted biological function: transferring of interferon blast-transforming activity to albebetin. Protein Engineering. 1996b;9(2):195-201. DOI: 10.1093/protein/9.2.195

14. Fedorov A.N., Dolgikh D.A., Chemeris V.V., Chernov B.K., Finkelstein A.V., Schulga A.A., Alakhov Yu.B., Kirpichnikov M.P., Ptitsyn O.B., De novo design, synthesis and study of albebetin, a polypeptide with a predetermined three-dimensional structure: Probing the structure at the nanogram level. Journal of Molecular Biology. 1992;225(4):927-931. DOI: 10.1016/0022-2836(92)90092-X

15. Gerasimova N.S., Volokh O.I., Pestov N.A., Armeev G.A., Kirpichnikov M.P., Shaytan A.K., Sokolova O.S., Studitsky V. M. Structure of an intranucleosomal DNA loop that senses DNA damage during transcription. Cells. 2022;11(17):2678. DOI: 10.3390/cells11172678

16. Ilina E.N., Solopova O.N., Balabashin D.S., Larina M.V., Aliev T.K., Grebennikova T.V., Losich M.A., Zaykova O.N., Sveshnikov P.G., Dolgikh D.A., Kirpichnikov M.P. Generation and characterization of a neutralizing monoclonal antibody against rabies virus. Russian Journal of Bioorganic Chemistry. 2018;44(6):695-704. DOI: 10.1134/S1068162019010072

17. Kirpichnikov M.P. Mechanisms of interaction of repressor proteins with DNA (Mekhanizmy vzaimodeystviya repressornykh belkov s DNK) [dissertation]. Moscow; 1987. [in Russian]

18. Kirpichnikov M.P., Hahn K.D., Buck F., Rüterjans H., Chernov B.K., Kurochkin A.V., Skryabin K.G., Bayev A.A. 1H NMR study of the interaction of bacteriophage lambda Cro protein with the OR3 operator. Evidence for a change of the conformation of the OR3 operator on binding. Nucleic Acids Research. 1984;12(8):3551-3561. DOI: 10.1093/nar/12.8.3551

19. Kirpichnikov M.P., Yartzev A.P., Minchenkova L.E., Chernov B.K., Ivanov V.I. The absence of non-local conformational changes in OR3 operator DNA on complexing with the cro repressor. Journal of Biomolecular Structure and Dynamics. 1985;3(3):529-536. DOI: 10.1080/07391102.1985.10508440

20. Lyubitelev A.V., Kirpichnikov M.P., Studitsky V.M. The role of linker histones in carcinogenesis. Russian Journal of Bioorganic Chemistry. 2021;47:278-287. DOI: 10.1134/S1068162021010143

21. Lyubitelev A.V., Kudryashova K.S., Mikhaylova M.S., Malyuchenko N.V., Chertkov O.V., Studitsky V.M., Feofanov A.V., Kirpichnikov M.P. Change in linker DNA conformation upon histone H1.5 binding to nucleosome: Fluorescent microscopy of single complexes. Moscow University Biological Sciences Bulletin. 2016;71:108-113. DOI: 10.3103/S0096392516020061

22. Lyukmanova E.N., Shenkarev Z.O., Shulepko M.A., Mineev K.S., D’Hoedt D., Kasheverov I.E., Filkin S.Y., Krivolapova A.P., Janickova H., Dolezal V., Dolgikh D.A., Arseniev A.S., Bertrand D., Tsetlin V.I., Kirpichnikov M.P. NMR structure and action on nicotinic acetylcholine receptors of water-soluble domain of human LYNX1. Journal of Biological Chemistry. 2011;25;286(12):10618-10627. DOI: 10.1074/jbc.m110.189100

23. [On the speech of Academician of the Russian Academy of Sciences M.P. Kirpichnikov at the round table within the framework of the VIII Congress of the Vavilov Society of Geneticists and Breeders]. Saratov National Research State University named after N. G. Chernyshevsky: [website]. 2024. [in Russian]. URL: https://www.sgu.ru/node/236211/. Дата публикации: 17.06.2024.

24. Petrovskaya L.E., Siletsky S.A., Mamedov M.D., Lukashev E.P., Balashov S.P., Dolgikh D.A., Kirpichnikov M.P. Features of the mechanism of proton transport in ESR, retinal protein from Exiguobacterium sibiricum. Biochemistry (Moscow). 2023;88:1544-1554. DOI: 10.1134/S0006297923100103

25. The engine of modern bioengineering: Academician Mikhail Kirpichnikov’s anniversary (Dvigatel sovremennoy bioinzhenerii: yubiley akademika Mikhaila Kirpichnikova). Scientific Russia: Electronic Periodical: [website]. 2025. [in Russian]. URL: https://scientificrussia.ru/articles/dvigatel-sovremennoj-bioinzenerii-ubilej-akademika-mihaila-kirpicnikova. Дата публикации: 09.11.2025.

26. Valieva M.E., Armeev G.A., Kudryashova K.S., Gerasimova N.S., Shaytan A.K., Kulaeva O.I., McCullough L.L., Formosa T., Georgiev P.G., Kirpichnikov M.P., Studitsky V.M., Feofanov A.V. Large-scale ATP-independent nucleosome unfolding by a histone chaperone. Nature Structural & Molecular Biology. 2016;23(12):1111-1116. DOI: 10.1038/nsmb.3321