Plant genetic resources with the basics of molecular genetics and breeding (on the results of the Additional Professional Education Program, St. Petersburg)

As part of the development program for the National Plant Genetic Resources Center, established at VIR by Decree of the President of the Russian Federation No. 44 dated February 8, 2022, an additional professional education program (qualification improvement program) entitled “Plant Genetic Resources (with the Basics of Molecular Genetics and Breeding)” was implemented in 2023. The program included five instructional and methodological modules: “Plant Genetic Resource Collections as Raw Material for Breeding. Collection Management”; “Plant Taxonomy and Herbarium Collection Management”; “Information Systems in the Work with Plant Genetic Resources”; “Molecular Genetics Methods in the Work with Plant Genetic Resources”; “Biotechnological Methods in the Work with Plant Genetic Resources”. The aim of these modules was to build theoretical and practical skills in the field of plant genetic resources diversity, modern molecular genetics technologies, and agricultural biotechnology in plant breeding. Fifty participants, selected on a competitive basis after reviewing their resumes and motivation letters, joined the program. They represented 18 scientific, research, and higher education institutions from 14 regions of the Russian Federation: Astrakhan, Kirov, Moscow, Novosibirsk, Omsk, Samara, Sverdlovsk and Tver Provinces, Altai, Krasnoyarsk, Primorsky, Stavropol and Khabarovsk Territories, and the Republic of Bashkortostan.

1. Agakhanov M.M., Grigoreva E.A., Potokina E.K., Ulianich P.S., Ukhatova Yu.V. Genome assembly of Vitis rotundifolia Michx. using third-generation sequencing (Oxford Nanopore Technologies). Proceedings on Applied Botany, Genetics and Breeding. 2021;182(2):63-71. [in Russian]. DOI: 10.30901/2227-8834- 2021-2-63-71

2. Bespalova E.S., Agakhanov M.M., Arkhimandritova S.B., Erastenkova M.V., Ukhatova Yu.V. Sanitization of potato varieties from the VIR collection against viruses. Proceedings on Applied Botany, Genetics and Breeding. 2020;181(4):164-172. [in Russian]. DOI: 10.30901/2227-8834-2020-4-164-172

3. Bespalova E.S., Ukhatova Yu.V., Volkova N.N., Oves E.V., Gaitova N.A., Gavrilenko T.A. Investigation of the postcryogenic regeneration ability of potato varieties under different cultivation conditions. Vavilov Journal of Genetics and Breeding. 2019;23(3):281-286. [in Russian]. DOI: 10.18699/VJ19.500

4. Chukhina I.G., Miftakhova S.R. Russian translation of the International Code of Nomenclature for Cultivated Plants. Proceedings on Applied Botany, Genetics and Breeding. 2022;183(3):183-187. [in Russian]. DOI: 10.30901/2227-8834-2022-3-183-187

5. Dunaeva S. E., Krasovskaya L.S., Gavrilenko T.A. Ex situ conservation of Rubus L. (Rosaceae) genetic resources (a review). Proceedings on Applied Botany, Genetics and Breeding. 2022;183(1):236-253. [in Russian]. DOI: 10.30901/2227- 8834-2022-1-236-253

6. Efremova O.S., Volkova N.N., Rybakov D.А., Lisitsyna O.V., Ozerski P.V., Gavrilenko T.A. Development of the potato cryocollection preserved in the VIR cryobank. Proceedings on Applied Botany, Genetics and Breeding. 2023;184(3):9-20. [in Russian]. DOI: 10.30901/2227-8834-2023-3-9-20

7. Gavrilenko T.A., Dunaeva S.E., Tikhonova O.A., Chukhina I.G. New approaches to registration and conservation of domestic cultivars of berry crops in the VIR Genebank on the example of red raspberry and black currant. Plant Biotechnology and Breeding. 2022;5(4):24-38. [in Russian]. DOI: 10.30901/2658-6266-2022-4-o5

8. Gavrilenko T.A., Shvachko N.A., Volkova N.N., Ukhatova Yu.V. A modified droplet vitrification method for cryopreservation of shoot tips from in vitro potato plants. Vavilov Journal of Genetics and Breeding. 2019;23(4):422-429. [in Russian]. DOI: 10.18699/VJ19.505

9. Gavrilova V.A., Makarova L.G., Stupnikova T.G., Alpatieva N.V., Kuznetsova E.B., Anisimova I.N. The trait-specific collection of large-seeded sunflower at VIR: a source for breeding cultivars and hybrids. Proceedings on Applied Botany, Genetics and Breeding. 2023;184(4):64-78. [in Russian]. DOI: 10.30901/2227-8834-2023-4-64-78

10. Kamnev А.М., Dunaeva S.E., Nevostrueva E.Yu., Kuzmina A.A., Gavrilenko Т.А., Chukhina I.G. Nomenclatural standards of raspberry cultivars bred by the Sverdlovsk Horticultural Breeding Station and Novosibirsk Zonal Gardening Station. Vavilovia. 2022a;5(4):13-38. [in Russian]. DOI: 10.30901/2658-3860-2022-4-о3

11. Kamnev А.М., Dunaeva S.Е., Volkova N.N., Lisitsyna O.V., Gavrilenko Т.А. Cryopreservation of raspberry cultivar accessions bred in Russia from the VIR in vitro collection. Plant Biotechnology and Breeding. 2022b;5(1):17-27. [in Russian]. DOI: 10.30901/2658-6266-2022-1-o2

12. Khlestkina E.K. Genetic resources in Russia: from collections to bioresource centers. Proceedings on Applied Botany, Genetics and Breeding. 2022;183(1):9-30. [in Russian]. DOI: 10.30901/2227-8834-2022-1-9-30

13. Khlestkina E.K., Ukhatova Y.V., Shipilina L.Y., Zavarzin A.A. Genetic resources and genetic technologies for the development of the Northern Territories: on the results of the Second Conference (March 13–15, 2023). Plant Biotechnology and Breeding. 2023;6(1):32-38. [in Russian]. DOI: 10.30901/2658-6266-2023-1-o5

14. Kornyukhin D.L., Artemyeva A.M. Breeding value of leafy and root turnip samples from the VIR collection. Vegetable Crops of Russia. 2022;(1):12-18. [in Russian]. DOI: 10.18619/2072-9146-2022-1-12-18

15. Korshikova E.S., Ershova K.M., Moksheninova Yu.A., Ukhatova Yu.V. Efficiency of in vitro culture techniques applied to soybean (Glycine max (L.) Merr.) accessions from the VIR collection. Proceedings on Applied Botany, Genetics and Breeding. 2021;182(4):137-142. [in Russian]. DOI: 10.30901/2227-8834-2021-4-137-142

16. Loskutov I.G., Blinova E.V., Gnutikov A.A. The collection of oat genetic resources held by VIR as a source of information on the history of cultivation and taxonomy of the genus, and breeding trends (a review). Proceedings on Applied Botany, Genetics and Breeding. 2023;184(1):225-238. [in Russian]. DOI: 10.30901/2227-8834-2023-1-225-238

17. Loskutov I.G, Novikova L.Yu., Kovaleva O.N., Ivanova N.N., Blinova E.V., Belskaya G.V. Ecological-geographic approaches to the study of genetic diversity of barley and oat from the VIR collection. Ecological genetics. 2020;18(1):89-102. [in Russian]. DOI: 10.17816/ecogen16128

18. Mironenko N.V., Rogozina E.V., Gurina A.A., Khiutti А.V., Chalaya N.A., Afanasenko O.S. Wild relatives and interspecific hybrids of potato as source materials in breeding for resistance to golden nematode. Proceedings on Applied Botany, Genetics and Breeding. 2020;181(4):173-184. [in Russian]. DOI: 10.30901/2227-8834-2020-4-173-184

19. Novikova L.Yu. Information system for assessing the adaptability of grape varieties to climate change. Scientific Works of the North Caucasus Federal Scientific Center for Horticulture, Viticulture, and Winemaking. 2019;24:67-73. [in Russian]. DOI: 10.30679/2587-9847-2019-24-67-73

20. Oskina N.A., Rybakov D.А., Shanina E.P., Lisitsyna O.V., Chukhina I.G., Gavrilenko T.А. An integrated approach to the registration and preservation of a cultivar gene pool in the VIR genebank exemplified in cultivars bred by the Ural Federal Agrarian Research Center of the Ural Branch of the Russian Academy of Sciences. Plant Biotechnology and Breeding. 2023;6(2):5-26. [in Russian]. DOI: 10.30901/2658-6266-2023-2-o4

21. Pavlov An.V., Porokhovinova E.A., Brutch N.B., Pavlov Al.V., Verzhuk V.G. The effect of cryopreservation in liquid nitrogen on the viability of flax seeds. Proceedings on Applied Botany, Genetics and Breeding. 2023;184(1):9-20. [in Russian]. DOI: 10.30901/2227-8834-2023-1-9-20

22. Rybakov D.А., Cheremisin A.I., Аntonova O.Yu., Chukhina I.G., Gavrilenko T.А. Nomenclatural standards and genetic passports of potato cultivars bred by the Omsk Agrarian Research Center. Plant Biotechnology and Breeding. 2022;5(4):6-23. [in Russian]. DOI: 10.30901/2658-6266-2022-4-o4

23. Semenova E.V., Boyko A.P., Novikova L.Y., Vishnyakova M.A. Phenotypic traits differentiating the genetic resources of pea (Pisum sativum L.) by the type of use. Vavilov Journal of Genetics and Breeding. 2022;26(7):599-608. [in Russian]. DOI: 10.18699/VJGB-22-74

24. Shamanin V.P., Pоtоtskaya I.V., Esse S.A., Gladkih M.S., Shepelev S.S., Zuev E.V., Vinichenko N.A., Koksel H., Morgounov A.I. Wheat landraces as sources of high grain quality and nutritional properties. Proceedings on Applied Botany, Genetics and Breeding. 2023;184(4):103-115. [in Russian]. DOI: 10.30901/2227-8834-2023-4-103-115

25. Talovina G.V., Zhidekhina T.V., Dunaeva S.E., Gur’eva I.V., Rodiukova O.S., Klimenko N.S., Kuzmina E.V., Gavrilenko T.А. Nomenclatural standards of black currant cultivars bred by the I.V. Michurin Federal Scientific Center. Vavilovia. 2023;6(2):3-32. [in Russian]. DOI: 10.30901/2658-3860-2023-2-o2

26. Tikhonova O.A., Gavrilova O.A., Radchenko E.A., Verzhuk V.G., Pavlov A.V. Viability of black currant pollen before and after cryopreservation in liquid nitrogen, and its morphological features. Proceedings on Applied Botany, Genetics and Breeding. 2020;181(3):110-119. [in Russian]. DOI: 10.30901/2227-8834-2020-3-110-119

27. Ukhatova Yu.V., Erastenkova M.V., Korshikova E.S., Krylova E.A., Mikhailova A.S., Semilet T.V., Tikhonova N.G., Shvachko N.A., Khlestkina E.K. Improvement of crops using the CRISPR/Cas system: New target genes. Molecular Biology. 2023;57(3):387-410 [in Russian]. DOI: 10.31857/S0026898423030151.

28. Verzhuk V., Eremin V., Gasanova T., Eremina O., Novikova L., Filipenko G., Sitnikov M., Pavlov A. Post-cryogenic viability of peach (Persica vulgaris Mill.) dormant buds from the VIR genetic collection. Agriculture. 2023;13(1):111). DOI: 10.3390/agriculture13010111.

29. Verzhuk V.G., Erastenkova M.V., Khokhlenko A.A., Agakhanov M.M., Kislin E.N., Ukhatova Yu.V. Evaluation of regenerative capacity of grape (Vitis vinifera L.) and red currant (Ribes rubrum L.) accessions in the culture in vitro for the development of VIR cryocollection. Magarach. Viticulture and Winemaking. 2022;24(3):214-218. [in Russian]. DOI: 10.34919/IM.2022.24.3.003

30. Vishnyakova M.A., Aleksandrova T.G., Buravtseva T.V. Burlyaeva M.O., Egorova G.P., Semenova E.V., Seferova I.V., Suvorova G.N. Species diversity of the VIR collection of grain legume genetic resources and its use in domestic breeding. Proceedings on Applied Botany, Genetics and Breeding 2019;180(2):109-123. [in Russian]. DOI: 10.30901/2227-8834-2019-2-109-123